HTLV screening of blood donors utilizing chemiluminescence immunoassay 

immunograde

Scientific software program of Chemiluminescence Microparticle Immunoassay for SARS-CoV-2 an an infection prognosis

Background: The unsatisfactory accuracy and functionality of precise time RT-PCR depends on quite a few unavoidable causes, which might’t meet the requires for COVID-19 prognosis.
Methods: 206 serum samples have been collected from victims who’ve been dealt with inside the Regular Hospital of the Central Theater Command of the PLA between January 18 and April 4, 2020. 270 serum samples from healthful blood donors have been used as administration. IgM and entire antibodies (Ab) in the direction of SARS-CoV-2 have been detected by Chemiluminescence Microparticle Immunoassay (CMIA).
Outcomes: Among the many many 206 victims, the optimistic worth of IgM and Ab have been 149/206 (72.3 %) and 187/206 (90.8 %), respectively. And the specificity of IgM and Ab detection have been 99.3 % and 98.9 %, respectively.
The sensitivity of CMIA for Ab detection was significantly bigger than that of IgM. An increase of the optimistic worth and S/CO price for detecting IgM and Ab accompanied with the rising of days post-disease onset (d.p.o.) have been observed.
The optimistic worth of Ab detected by CMIA elevated shortly after 7 d.p.o., whereas that of IgM was clearly elevated after 14 d.p.o.. In addition to, the age and gender of these victims did not impact the seroconversion and titer of antibodies all through the complete course. The disease-severity of victims had no impression on the seroconversion of antibodies. Nonetheless, the very important victims possessed a lots bigger antibody titers than the no-critical cases after 14 d.p.o..
Conclusions: The CMIA can current essential complementation to nucleic acid assay and help to spice up the accuracy and functionality of prognosis of SARS-CoV-2 an an infection.
Key phrases: Antibody; Chemiluminescence immunoassay; SARS-CoV-2; Serodiagnosis.

HTLV screening of blood donors using chemiluminescence immunoassay in three principal provincial blood services of China

Background: Human T-celllymphotropic virus (HTLV) stays a big safety concern for blood gives. No matter many HTLV optimistic cases being reported in southeastern China, the detection of HTLV has not been prioritized in routine blood screening.
Furthermore, info on the prevalence of HTLV an an infection amongst blood donors may also be restricted. The goal of this look at was to investigate the prevalence of HTLV amongst blood donors in three Chinese language language provinces by their advisor blood services, to evaluate the feasibility of chemiluminescence immunoassay (CLIA) for blood screening.
Methods: From November 2018 to March 2019, blood plasma samples have been collected from Hebei, Changsha, and Shenzhen blood services and have been screened for the HTLV-1\/2 antibody using a CLIA and enzyme-linked immunosorbent assay (ELISA). This was adopted by confirmatorytests using INNO-LIA HTLV I/II.
Outcomes: A whole of 59,929 blood donations have been collected and screened for HTLV-1\/2.
The reactive worth of CLIA and ELISA amongst donations inside the Shenzhen blood coronary heart (0.0943%, 27/28,621) was bigger than Hebei (0.0248%, 4/16,144), and Changsha (0.0198%, 3/15,164) (p < 0.05).
After affirmation, Three samples have been confirmed as indeterminate for HTLV antibodies, and only one sample from the Shenzhen blood coronary heart was confirmed as HTLV-1.
The overall prevalence of HTLV-1\/2 was 1.67 per 100,000 (1/59,929). The HTLV-infected blood obtained right here from a 32-year-old first-time female donor with a highschool diploma, who belonged to the SHE ethnic minority and was born inside the Fujian province.
Conclusions: In summary, the overall prevalence of HTLV-1\/2 amongst blood donors inside the three blood services in China stays comparatively low.
Nonetheless, blood donations with optimistic or indeterminate outcomes for HTLV antibodies reminded us of the importance of HTLV screening amongst blood donors in China.
Key phrases: Blood donors; Blood screening; Chemiluminescence immunoassay (CLIA); Human T-cell lymphotropic virus (HTLV); Prevalence.
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Scientific Utility of a Extraordinarily Delicate Lateral Stream Immunoassay as determined by Titer Analysis for the Detection of anti-SARS-CoV-2 Antibodies on the Stage-of-Care

Coronavirus sickness 2019 (COVID-19), introduced on by the intense acute respiratory syndrome coronavirus-2 (SARS-CoV-2), turned a pandemic in early 2020.
Lateral transfer immunoassays for antibody testing have been seen as an inexpensive and shortly deployable method for determining earlier an an infection with SARS-CoV-2; nonetheless, these assays have confirmed unacceptably low sensitivity.
We report on 9 lateral transfer immunoassays for the time being on the market and consider their titer sensitivity in serum to a best-practice enzyme-linked immunosorbent assay (ELISA) and viral neutralization assay.
For a small group of PCR-positive, we found two lateral transfer immunoassay devices with titer sensitivity roughly equal to the ELISA; these devices have been optimistic for all PCR-positive victims harboring SARS-CoV-2 neutralizing antibodies.
Thought of one in all these devices was deployed in Northern Italy to examine its sensitivity and specificity in a real-world medical setting. Using the system with fingerstick blood on a cohort of 27 hospitalized PCR-positive victims and seven hospitalized controls, ROC curve analysis gave AUC values of 0.7646 for IgG.
For comparability, this assay was moreover examined with saliva from the an identical affected individual inhabitants and confirmed decreased discrimination between cases and controls with AUC values of 0.6841 for IgG.
Furthermore, all through viral neutralization testing, one affected individual was discovered to harbor autoantibodies to ACE2, with implications for a manner immune responses are profiled.
We current proper right here by a proof-of-concept look at that these lateral transfer devices is likely to be as analytically delicate as ELISAs and adopted into hospital protocols; nonetheless, additional enhancements to these devices keep compulsory sooner than their medical deployment.

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K371-100 each
EUR 614.4

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K372-100 each
EUR 614.4

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EUR 628.92
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Human Complement C3 AssayLite Fluorescent Immunoassay Kit

FEC2101 96 Well Plate
EUR 475.2

Human Complement C4 AssayLite Fluorescent Immunoassay Kit

FEC2102 96 Well Plate
EUR 475.2

Human Fibrinogen (FBG) AssayLite Fluorescent Immunoassay Kit

FEF1040 96 Well Plate
EUR 475.2

Rat Fth1(Ferritin heavy chain) ELISA Kit

ER0090 96T
EUR 681.12
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Rattus;Sensitivity: 3.75 ng/ml

Human ACE(Angiotensin I Converting Enzyme) ELISA Kit

EH0026 96T
EUR 628.92
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.469 ng/ml

Human ACE2(Angiotensin-converting enzyme 2) ELISA Kit

EH0027 96T
EUR 628.92
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.234 ng/ml

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EH1264 96T
EUR 681.12
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Human APEH(Acylamino-acid-releasing enzyme) ELISA Kit

EH1573 96T
EUR 681.12
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Human ECE1(Endothelin-converting enzyme 1) ELISA Kit

EH2454 96T
EUR 628.92
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 18.75pg/ml

Human UBE2C(Ubiquitin Conjugating Enzyme E2C) ELISA Kit

EH3939 96T
EUR 628.92
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.094 ng/ml

Recombinant human Glycogen debranching enzyme

P2412 100ug Ask for price
Description: Recombinant protein for human Glycogen debranching enzyme

Mouse Albumin AssayLite Fluorescent Immunoassay Kit

FEMA2201 96 Well Plate
EUR 475.2

Rat Albumin AssayLite Fluorescent Immunoassay Kit

FERA2201 96 Well Plate
EUR 475.2

CaspSELECT? Caspase-3 Immunoassay Kit (Fluorometric)

K163-100 each
EUR 901.2

Immunoassay Stabilizer (BSA free)

85R-103BF 1 liter
EUR 577.2
Description: BSA Free Stabilizing buffer for use in immunoassays

anti- Ferritin light chain antibody

FNab03079 100µg
EUR 606.3
Description: Antibody raised against Ferritin light chain

Human Ferritin ELISA Kit

E-80F 1 x 96 well plate
EUR 462

Human Ferritin ELISA kit

E01F0010-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Human Ferritin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Ferritin ELISA kit

E01F0010-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Human Ferritin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Ferritin ELISA kit

E01F0010-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Human Ferritin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Ferritin ELISA kit

LF-EK0149 1×96T
EUR 723.6

Ferritin (human) ELISA Kit

K7420-100 each
EUR 966

Human UBE2A(Ubiquitin-conjugating enzyme E2 A) ELISA Kit

EH1106 96T
EUR 628.92
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.188 ng/ml

Human SAE1(SUMO-activating enzyme subunit 1) ELISA Kit

EH2266 96T
EUR 681.12
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.094 ng/ml

(1-Kit )11,12-EET/DHET Immunoassay Kit

DH5 1 Kit
EUR 368.4

Human Alpha-1-Antitrypsin AssayLite Fluorescent Immunoassay Kit

FEA5101 96 Well Plate
EUR 475.2

Recombinant human Acylamino-acid-releasing enzyme

P1835 100ug Ask for price
Description: Recombinant protein for human Acylamino-acid-releasing enzyme

Rat Ace(Angiotensin-converting enzyme) ELISA Kit

ER0094 96T
EUR 628.92
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Rattus;Sensitivity: 0.188 ng/ml

Mouse Ide(Insulin-degrading enzyme) ELISA Kit

EM0694 96T
EUR 681.12
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Mus ;Sensitivity: 0.188 ng/ml

Human CYP11A1(Cholesterol side-chain cleavage enzyme, mitochondrial) ELISA Kit

EH1103 96T
EUR 681.12
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.188 ng/ml

Human UBA3(NEDD8-activating enzyme E1 catalytic subunit) ELISA Kit

EH1150 96T
EUR 681.12
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.094 ng/ml

Human BACE1(Beta-site APP Cleaving Enzyme 1) ELISA Kit

EH2687 96T
EUR 628.92
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.375 ng/ml

Human UBE1L(Ubiquitin Activating Enzyme E1 Like Protein) ELISA Kit

EH3938 96T
EUR 628.92
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 46.875pg/ml

Human Ferritin depleted/stripped serum (Ferritin

1810-FDS-10 10 ml Ask for price

Human Ferritin depleted/stripped serum (Ferritin

1810-FDS-1000 1000 ml Ask for price

Recombinant human NADP-dependent malic enzyme, mitochondrial

P2328 100ug Ask for price
Description: Recombinant protein for human NADP-dependent malic enzyme, mitochondrial

Anti-SARS-CoV-2 Neutralization Antibody Test Kit (Enzyme-linked immunosorbent assay)

CP04012 96T/5*96T Ask for price
Description: It is a semi quantitative competition ELISA kit to detect the Anti-SARS-CoV-2 Neutralization Antibody in human serum or plasma. The micro test plate provided in this kit is pre-coated with recombinant human ACE2. During the reaction, the SARS-CoV-2 neutralization antibody in the standard & sample diluent pretreated samples or controls competes with a fixed amount of human ACE2 on the solid phase supporter for sites on the Horseradish peroxidase (HRP) conjugated recombinant SARS-CoV-2 RBD fragment (HRP-RBD). After 37℃incubation, the unbound HRP-RBD as well as any HRP-RBD bound to non-neutralization antibody will be captured on the plate and eventually form the ACE2-RBD-HRP complex, while the circulating neutralization antibodies HRP-RBD complexes remain in the supernatant and are removed during washing. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm.

QuickTiter Adenovirus Titer Immunoassay Kit, Trial Size

VPK-109-T 20 assays
EUR 448.8
Description: Accurate measurement of adenovirus titer is critical for gene delivery. Traditional plaque-forming unit (PFU) assays are long and suffer from high inter-assay variability. The QuickTiter Adenovirus Titer Immunoassay Kit provide a quick, complete system to functionally titer virus infectivity. The assay recognizes all 41 serotypes of adenovirus, and can be used with any adenovirus system that can amplify in HEK 293 cells.

Ferritin ELISA kit

55R-1881 1 kit
EUR 891.6
Description: ELISA kit for the detection of Ferritin in the research laboratory

Ferritin ELISA kit

55R-ORG5FE 96 wells
EUR 415.2
Description: ELISA kit for the detection of Ferritin in the research laboratory

Frit Kit

FRIT-KIT 1each
EUR 148.8
Description: Kit to create frits in capillaries. Includes formamide, Kasil-1, Kasil-1624 and a cleaving tool.

Human Ceruloplasmin and Haptoglobin AssayLite Multiplex Fluorescent Immunoassay Kit

FH100311M2 96 Well Plate
EUR 1083.6

Rat Ace2(Angiotensin-converting enzyme 2) ELISA Kit

ER0609 96T
EUR 681.12
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Rattus;Sensitivity: 0.094 ng/ml

Rat IRE1(Inositol requiring enzyme 1) ELISA Kit

ER1646 96T
EUR 681.12
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Rattus ;Sensitivity: 28.1pg/ml

Rabbit ACE(Angiotensin I Converting Enzyme) ELISA Kit

ERB0001 96T
EUR 681.12
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Rabbit;Sensitivity: 0.188 ng/ml

Mouse ACE(Angiotensin I Converting Enzyme) ELISA Kit

EM0011 96T
EUR 628.92
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Mus ;Sensitivity: 0.188 ng/ml

Mouse ECE1(Endothelin Converting Enzyme 1) ELISA Kit

EM0995 96T
EUR 628.92
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Mus ;Sensitivity: 9.375pg/ml

Monkey ACE(Angiotensin I Converting Enzyme) ELISA Kit

EMK0001 96T
EUR 681.12
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Ape;Sensitivity: 0.094 ng/ml

Sheep ACE(Angiotensin I Converting Enzyme) ELISA Kit

ESH0001 96T
EUR 681.12
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Sheep;Sensitivity: 0.469 ng/ml

Up-converting Phosphor immunoassay analyzer

abx095263-1Machine 1 Machine
EUR 7326

Human Ferritin (FE) ELISA Kit

20-abx151505
  • EUR 6373.20
  • EUR 3404.40
  • EUR 801.60
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Human Ferritin (FE) CLIA Kit

abx195617-96tests 96 tests
EUR 990

Human Ferritin (FE) ELISA Kit

abx253212-96tests 96 tests
EUR 764.4

Human Ferritin (FE) CLIA Kit

20-abx190110
  • EUR 9493.20
  • EUR 5058.00
  • EUR 1167.60
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Human ferritin,FE ELISA Kit

201-12-1703 96 tests
EUR 528
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

Human ferritin, FE ELISA Kit

CSB-E05187h-24T 1 plate of 24 wells
EUR 198
Description: Quantitative sandwich ELISA kit for measuring Human ferritin, FE in samples from serum, plasma, cell culture supernates, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Human ferritin, FE ELISA Kit

1-CSB-E05187h
  • EUR 546.00
  • EUR 3794.40
  • EUR 2034.00
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
Description: Quantitative sandwich ELISA kit for measuring Human ferritin, FE in samples from serum, plasma, cell culture supernates, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Human ferritin,FE ELISA Kit

CN-04117H1 96T
EUR 520.8

Human ferritin,FE ELISA Kit

CN-04117H2 48T
EUR 340.8

Human H ferritin ELISA kit

E01H0243-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Human H ferritin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human H ferritin ELISA kit

E01H0243-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Human H ferritin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human H ferritin ELISA kit

E01H0243-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Human H ferritin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Ferritin ELISA Kit (Human) (OKCD06395)

OKCD06395 96 Wells
EUR 574.8
Description: Description of target: This gene encodes the light subunit of the ferritin protein. Ferritin is the major intracellular iron storage protein in prokaryotes and eukaryotes. It is composed of 24 subunits of the heavy and light ferritin chains. Variation in ferritin subunit composition may affect the rates of iron uptake and release in different tissues. A major function of ferritin is the storage of iron in a soluble and nontoxic state. Defects in this light chain ferritin gene are associated with several neurodegenerative diseases and hyperferritinemia-cataract syndrome. This gene has multiple pseudogenes.;Species reactivity: Human;Application: ELISA;Assay info: ;Sensitivity: < 0.061ng/mL

Human Ferritin AssayMax ELISA Kit

EF2003-1 96 Well Plate
EUR 475.2

Human Carcinoembryonic Ferritin ELISA kit

E01C0729-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Human Carcinoembryonic Ferritin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Carcinoembryonic Ferritin ELISA kit

E01C0729-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Human Carcinoembryonic Ferritin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

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