Photoelectrochemical immunoassay platform based on MoS 2 nanosheets built-in with gold nanostars for neuron-specific enolase assay
MoS2 nanosheets had been prepared by exfoliating MoS2 bulk crystals with ultrasonication in N-methylpyrrolidone and had been built-in with gold nanostars (AuNS) to fabricate an AuNS/MoS2 nanocomposite.
All nanomaterials had been characterised by transmission electron microscope, scanning electron microscope, ultraviolet-visible spectroscopy, X-ray diffraction, and X-ray photoelectron spectroscopy.
A0uNS/MoS2 nanocomposites had been coated onto a glassy carbon electrode (GCE) ground to assemble a nanointerface for immobilizing neuron-specific enolase antibody (anti-NSE) thus forming a photoelectrochemical immunoassay system.
AuNS can significantly promote the photoelectric conversion of MoS2 nanosheets bettering the effectivity for a photoelectrochemical assay.
Being illuminated with white gentle LED and controlling the potential at 0.05 V (vs. SCE), the photocurrent generated from anti-NSE(BSA)/AuNS/MoS2/GCE using 0.15 mol L-1 ascorbic acid as electron donor is likely to be recorded with amperometry and used as an output signal for NSE quantitative assay.
Beneath optimized experimental circumstances, the photocurrent variation for the affinity-binding NSE is proportional to the logarithm of NSE focus throughout the differ 5.Zero pg mL-1 to 1.5 ng mL-1 with a detection prohibit of three.5 pg mL-1 (S/N = 3).
The practicability of the PEC immunoassay system was evaluated by determining NSE in scientific serum samples. The recoveries ranged from 93.Zero to 103% for the dedication of NSE in serum samples with an unusual addition methodology.
The PEC immunoassay system possesses good accuracy for determining NSE in precise samples. Graphical abstract.
Key phrases: Gold nanostar; MoS2 nanosheet; Neuron-specific enolase; Photoelectrochemical immunoassay.
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Troponin-I (Human Cardiac-Specific) Enzyme Immunoassay Test Kit |
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Rapid Visual Human Chorionic Gonadotropin (hCG) Enzyme Immunoassay Test Kit |
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Human Toxoplasma IgG (TOXO IgG) Rapid Test Kit |
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Human Toxoplasma IgG (TOXO IgG) Rapid Test Kit |
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Toxoplasma gondii IgG/IgM (T. gondii IgG/IgM) Rapid Test Kit |
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Goat Toxoplasma IgG,Toxoplasma IgG ELISA KIT |
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Goat Toxoplasma IgG, Toxoplasma IgG ELISA Kit |
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MBS1610038-96StripWells | MyBiosource | 96-Strip-Wells | EUR 475 |
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abx092078-100l | Abbexa | 100 µl | EUR 262.5 |
Human Cytomegalovirus IgG/IgM, Toxoplasma IgG/IgM, Rubella IgG (CMV + TOXO IgG/IgM, RV IgG) Rapid Test Kit |
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Dog and Cat Toxoplasma Gondi (TOXO Ab) Rapid Test Kit |
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Chicken Toxoplasma IgG ELISA Kit |
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Toxoplasma (TOX) IgG ELISA Kit |
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Toxoplasma (TOX) IgG ELISA Kit |
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Description: ELISA kit for the detection of Toxoplasma gondii IgG in the research laboratory |
Toxoplasma gondii IgG ELISA Kit |
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Description: Human Angiotensin I Converting Enzyme Chemiluminescent Immunoassay Kit |
Mouse Angiotensin I Converting Enzyme Chemiluminescent Immunoassay Kit |
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Description: Mouse Angiotensin I Converting Enzyme Chemiluminescent Immunoassay Kit |
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A microwell array structured ground plasmon resonance imaging gold chip for high-performance label-free immunoassay
Flooring plasmon resonance imaging (SPRi) offers a compelling methodology for high-throughput, real-time, and label-free biomolecular interaction analysis and immunoassays, nevertheless its effectivity suffers from restricted intrinsic sensitivity and low-contrast SPRi pictures.
Herein we report a high-performance SPRi chip that features patterned microwell array constructed by photolithography of adhesive polydopamine (PDA) skinny film on typical gold chip.
The chip permits for the facile constructing of region-defined sensing array on its ground with improved intrinsic SPRi sensitivity due to the intensified ground plasmon wave (SPW) throughout the microwells.
The immunoassay effectivity of the as-designed SPRi chip is evaluated by using anti-ochratoxin A (anti-OTA) monoclonal antibody as a model aim.
The outcomes current that this microwell array structured gold chip shows ca. 18%-32% elevated signal depth than the typical gold chip when detecting anti-OTA at fully completely different concentrations, and the noise stays on the similar diploma, displaying enhanced intrinsic sensitivity.
Within the meantime, this microwell-structured chip affords clear and high-contrast SPRi pictures with well-defined sensing areas, which enormously facilitates the extraction and quantitative analysis of detection indicators whereas successfully suppressing the disturbance from background areas.
Antibody response in direction of SARS-CoV-2 spike protein and nucleoprotein evaluated by 4 automated immunoassays and three ELISAs
Objectives: To search out out the antibody response in direction of SARS-CoV-2 spike protein and nucleoprotein using 4 automated immunoassays and three ELISAs for the detection of entire Ig antibodies (Roche) or IgG (Abbott, Diasorin, Snibe, Euroimmun, Mikrogen) in COVID-19 victims.
Methods: Sensitivity and dynamic growth to seropositivity had been evaluated in 233 samples from 114 victims with affordable, excessive, or essential COVID-19 confirmed with PCR on nasopharyngeal swab. Specificity was evaluated in 113 samples collected sooner than January 2020 along with 24 samples from victims with non-SARS coronavirus an an infection.
Outcomes: Sensitivity for all assays was 100% [95% confidence interval: 83.7-100] Three weeks after onset of indicators. Specificity varied between 94.7% [88.7-97.8] and 100% [96.1-100]. Calculated on the cut-offs that corresponded to a specificity of 95% and 97.5%,
Roche had the very best sensitivity (85.0% [79.8-89.0] and 81.1% [76.6-85.7], p <0.05 in addition to vs. Abbott).
Seroconversion occurred on frequent 2 days earlier for Roche entire Ig anti-N and the 2 IgG anti-N assays (Abbott and Euroimmun) than for the 2 IgG anti-S assays (Diasorin and Euroimmun) (≥50% seroconversion day 9-10 vs. day 11-12, and p <0.05 for % seropositive victims day 9-10 to 17-18).
There was no very important distinction throughout the IgG antibody time to seroconversion between essential and non-critical victims.
Conclusions: Seroconversion occurred inside Three weeks after onset of indicators with all assays and on frequent 2 days earlier for assays detecting IgG or entire Ig anti-N than for IgG anti-S. The specificity of assays detecting anti-N was equivalent to anti-S and superb in a troublesome administration inhabitants.
Toxoplasma IgG Enzyme Immunoassay Test Kit |
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HSV-2 IgG Enzyme Immunoassay Test Kit |
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Immunoglobulin E (IgE) Enzyme Immunoassay Test Kit |
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Luteinizing Hormone (LH) Enzyme Immunoassay Test Kit |
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Helicobacter Pylori IgG Enzyme Immunoassay Test Kit |
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Cytomegalovirus (CMV) IgG Enzyme Immunoassay Test Kit |
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Beta-2-Microglobulin Enzyme Immunoassay Test Kit |
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Cancer Antigen CA19-9 Enzyme Immunoassay Test Kit |
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GWB-EED29A | GenWay Biotech | ELISA_Kits | Ask for price |
Carcinoembryonic Antigen (CEA) Enzyme Immunoassay Test Kit |
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GWB-FB64BF | GenWay Biotech | ELISA_Kits | Ask for price |
Human Growth Hormone (HGH) Enzyme Immunoassay Test Kit |
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Prostate Specific Antigen (PSA) Enzyme Immunoassay Test Kit |
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Thyroid Stimulating Hormone (TSH) Enzyme Immunoassay Test Kit |
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Human Chorionic Gonadotropin (hCG) Enzyme immunoassay Test Kit |
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GWB-E3238F | GenWay Biotech | ELISA_Kits | Ask for price |
Breast Cancer Antigen CA15-3 Enzyme Immunoassay Test Kit |
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GWB-150A4D | GenWay Biotech | ELISA_Kits | Ask for price |
GWB-AFC61F-1U - Estradiol (E2) Enzyme Immunoassay Test Kit |
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Follicle-Stimulating Hormone (FSH) Enzyme Immunoassay Test Kit |
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GWB-44F874 | GenWay Biotech | ELISA_Kits | Ask for price |
GWB-5185FE-1U - HSV-2 IgG Enzyme Immunoassay Test Kit |
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GWB-5185FE-1U | Aviva Systems Biology | 96wells | EUR 329 |
Troponin-I (Human Cardiac-Specific) Enzyme Immunoassay Test Kit |
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GWB-83A61F | GenWay Biotech | ELISA_Kits | Ask for price |
High Sensitivity C-Reactive Protein (CRP) Enzyme Immunoassay Test Kit |
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GWB-C97162 | GenWay Biotech | ELISA_Kits | Ask for price |
GWB-892399-96W - Cytomegalovirus (CMV) IgG Enzyme Immunoassay Test Kit |
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GWB-892399-96W | Aviva Systems Biology | 96wells | EUR 329 |
Rapid Visual Human Chorionic Gonadotropin (hCG) Enzyme Immunoassay Test Kit |
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GWB-DAB550 | GenWay Biotech | ELISA_Kits | Ask for price |
ULTRASENSITIVE THYROID STIMULATING HORMONE (U-TSH) ENZYME IMMUNOASSAY TEST KIT |
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GWB-8BFFB0 | GenWay Biotech | ELISA_Kits | Ask for price |
GWB-DAB550-1U - Rapid Visual Human Chorionic Gonadotropin (hCG) Enzyme Immunoassay Test Kit |
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GWB-DAB550-1U | Aviva Systems Biology | 96Wells | EUR 219 |
Aldosterone Enzyme Immunoassay Kit |
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DEIABL242 | Creative Diagnostics | 96T | EUR 1026 |
Description: The Aldosterone Immunoassay kit is designed to quantitatively measure Aldosterone present in extracted serum and plasma, or in urine, extracted dried fecal samples, and tissue culture media samples. This kit measures total aldosterone in extracted serum or plasma and fecal samples. |
Progesterone Rapid Test Kit |
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100047 | Ring Biotechnology Co | 96T | EUR 100 |
Description: Milk Test |
Progesterone Rapid Test Kit |
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102205 | Ring Biotechnology Co | 96T | EUR 100 |
Description: Milk Test |
PG(Progesterone) ELISA Kit |
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EU0398 | FN Test | 96T | EUR 571.5 |
Description: Method of detection: Coated with Antigen, Competitive ELISA;Reacts with: General;Sensitivity: 0.188 ng/ml |
Sheep Progesterone ELISA Kit |
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ESH0086 | FN Test | 96T | EUR 681.12 |
Description: Method of detection: Coated with Antigen, Competitive ELISA;Reacts with: Sheep;Sensitivity: 0.188 ng/ml |
Dog Progesterone (cProg) Rapid Test Kit |
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abx472043-1096tests | Abbexa | 10 × 96 tests | Ask for price |
Dog Progesterone (cProg) Rapid Test Kit |
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abx472043-596tests | Abbexa | 5 × 96 tests | Ask for price |
Dog Progesterone (cProg) Rapid Test Kit |
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abx472043-96tests | Abbexa | 96 tests | EUR 312.5 |
Rat Pg(Progesterone) ELISA Kit |
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ER1255 | FN Test | 96T | EUR 571.5 |
Description: Method of detection: Coated with Antigen, Competitive ELISA;Reacts with: Rattus;Sensitivity: 0.188 ng/ml |
Mouse Pg(Progesterone) ELISA Kit |
|||
EM1281 | FN Test | 96T | EUR 571.5 |
Description: Method of detection: Coated with Antigen, Competitive ELISA;Reacts with: Mus ;Sensitivity: 0.188 ng/ml |
Bovine Pg(Progesterone) ELISA Kit |
|||
EB0016 | FN Test | 96T | EUR 681.12 |
Description: Method of detection: Coated with Antigen, Competitive ELISA;Reacts with: Cattle;Sensitivity: 0.188 ng/ml |
Monkey Pg(Progesterone) ELISA Kit |
|||
EMK0060 | FN Test | 96T | EUR 681.12 |
Description: Method of detection: Coated with Antigen, Competitive ELISA;Reacts with: Ape;Sensitivity: 0.188 ng/ml |
Sunlong Medical™ Mouse AXL Enzyme Immunoassay Kit |
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EL0123Mo-48T | Sunlong | 48T | EUR 398 |
Sunlong Medical™ Mouse AXL Enzyme Immunoassay Kit |
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EL0123Mo-96T | Sunlong | 96T | EUR 569 |
Chicken Pg(Progesterone) ELISA Kit |
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ECH0061 | FN Test | 96T | EUR 681.12 |
Description: Method of detection: Coated with Antigen, Competitive ELISA;Reacts with: Chicken;Sensitivity: 0.188 ng/ml |
Porcine Pg(Progesterone) ELISA Kit |
|||
EP0136 | FN Test | 96T | EUR 681.12 |
Description: Method of detection: Coated with Antigen, Competitive ELISA;Reacts with: Pig;Sensitivity: 0.188 ng/ml |
Rat Pgr(Progesterone receptor) ELISA Kit |
|||
ER0492 | FN Test | 96T | EUR 628.92 |
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Rattus;Sensitivity: 0.188 ng/ml |
Human PGR(Progesterone receptor) ELISA Kit |
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EH1449 | FN Test | 96T | EUR 628.92 |
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.188 ng/ml |
Canine Progesterone Rapid Test Card |
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C11541 | Ring Biotechnology Co | 15T | Ask for price |
Free Thyroxine (fT4) Enzyme Immunoassy Test Kit |
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GWB-9857BC | GenWay Biotech | ELISA_Kits | Ask for price |
Human Estrone-3-Glucuronide (E1G)Enzyme Immunoassay ELISA Kit |
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QY-E05423 | Qayee Biotechnology | 96T | EUR 433.2 |
Human Insulin Degrading Enzyme Chemiluminescent Immunoassay Kit |
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IHUIDEKTC | Innovative research | each | EUR 545 |
Description: Human Insulin Degrading Enzyme Chemiluminescent Immunoassay Kit |
Alpha-Fetoprotein (AFP) Enzyme Immunoassy Test Kit |
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GWB-C3BE7B | GenWay Biotech | ELISA_Kits | Ask for price |
Free Triiodothyronine (fT3) Enzyme Immunoassy Test Kit |
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GWB-AA1FE4 | GenWay Biotech | ELISA_Kits | Ask for price |
Human PIBF(Progesterone-induced-blocking factor) ELISA Kit |
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EH1818 | FN Test | 96T | EUR 681.12 |
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.469 ng/ml |
Human Angiotensin I Converting Enzyme Chemiluminescent Immunoassay Kit |
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IHUACEKTC | Innovative research | each | EUR 545 |
Description: Human Angiotensin I Converting Enzyme Chemiluminescent Immunoassay Kit |
Mouse Angiotensin I Converting Enzyme Chemiluminescent Immunoassay Kit |
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IMSACEKTC | Innovative research | each | EUR 545 |
Description: Mouse Angiotensin I Converting Enzyme Chemiluminescent Immunoassay Kit |
Human Angiotensin I Converting Enzyme 2 Chemiluminescent Immunoassay Kit |
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IHUACE2KTC | Innovative research | each | EUR 545 |
Description: Human Angiotensin I Converting Enzyme 2 Chemiluminescent Immunoassay Kit |
Canine Cortisol Test Kit (Fluorescence Lateral Flow Immunoassay) |
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HG017 | EnoGene | 10 Tests/Kit | EUR 63 |
Description: Contents: (1) 10 Tests/Kit (cassette,disposable dropper,desiccant) (2) 10 Assay buffer tubes (3) 10 Sterile swabs (4) 1 Instruction manual |
Cat Progesterone Immunomodulatory Binding Factor 1 ELISA Kit |
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MBS088264-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Rat Progesterone Immunomodulatory Binding Factor 1 ELISA Kit |
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MBS051379-10x96StripWells | MyBiosource | 10x96-Strip-Wells | EUR 6725 |
Rat Progesterone Immunomodulatory Binding Factor 1 ELISA Kit |
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MBS051379-48StripWells | MyBiosource | 48-Strip-Wells | EUR 550 |
Rat Progesterone Immunomodulatory Binding Factor 1 ELISA Kit |
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MBS051379-5x96StripWells | MyBiosource | 5x96-Strip-Wells | EUR 3420 |
Rat Progesterone Immunomodulatory Binding Factor 1 ELISA Kit |
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MBS051379-96StripWells | MyBiosource | 96-Strip-Wells | EUR 765 |
Goat Progesterone Immunomodulatory Binding Factor 1 ELISA Kit |
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MBS096750-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Fish Progesterone Immunomodulatory Binding Factor 1 ELISA Kit |
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MBS102234-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Duck Progesterone Immunomodulatory Binding Factor 1 ELISA Kit |
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MBS058507-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Cavy Progesterone Immunomodulatory Binding Factor 1 ELISA Kit |
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MBS074954-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Human Progesterone Immunomodulatory Binding Factor 1 ELISA Kit |
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MBS092623-10x96StripWells | MyBiosource | 10x96-Strip-Wells | EUR 6725 |
Human Progesterone Immunomodulatory Binding Factor 1 ELISA Kit |
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MBS092623-48StripWells | MyBiosource | 48-Strip-Wells | EUR 550 |
Human Progesterone Immunomodulatory Binding Factor 1 ELISA Kit |
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MBS092623-5x96StripWells | MyBiosource | 5x96-Strip-Wells | EUR 3420 |
Human Progesterone Immunomodulatory Binding Factor 1 ELISA Kit |
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MBS092623-96StripWells | MyBiosource | 96-Strip-Wells | EUR 765 |
Horse Progesterone Immunomodulatory Binding Factor 1 ELISA Kit |
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MBS100371-10x96StripWells | MyBiosource | 10x96-Strip-Wells | EUR 6725 |
Horse Progesterone Immunomodulatory Binding Factor 1 ELISA Kit |
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MBS100371-48StripWells | MyBiosource | 48-Strip-Wells | EUR 550 |
Horse Progesterone Immunomodulatory Binding Factor 1 ELISA Kit |
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MBS100371-5x96StripWells | MyBiosource | 5x96-Strip-Wells | EUR 3420 |
Horse Progesterone Immunomodulatory Binding Factor 1 ELISA Kit |
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MBS100371-96StripWells | MyBiosource | 96-Strip-Wells | EUR 765 |
Bovine Progesterone Immunomodulatory Binding Factor 1 ELISA Kit |
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MBS047884-10x96StripWells | MyBiosource | 10x96-Strip-Wells | EUR 6725 |
Bovine Progesterone Immunomodulatory Binding Factor 1 ELISA Kit |
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MBS047884-48StripWells | MyBiosource | 48-Strip-Wells | EUR 550 |
Bovine Progesterone Immunomodulatory Binding Factor 1 ELISA Kit |
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MBS047884-5x96StripWells | MyBiosource | 5x96-Strip-Wells | EUR 3420 |
Bovine Progesterone Immunomodulatory Binding Factor 1 ELISA Kit |
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MBS047884-96StripWells | MyBiosource | 96-Strip-Wells | EUR 765 |
Pigeon Progesterone Immunomodulatory Binding Factor 1 ELISA Kit |
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MBS059879-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Donkey Progesterone Immunomodulatory Binding Factor 1 ELISA Kit |
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MBS056695-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Rabbit Progesterone Immunomodulatory Binding Factor 1 ELISA Kit |
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MBS055418-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Canine Progesterone Immunomodulatory Binding Factor 1 ELISA Kit |
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MBS054074-10x96StripWells | MyBiosource | 10x96-Strip-Wells | EUR 6725 |
Canine Progesterone Immunomodulatory Binding Factor 1 ELISA Kit |
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MBS054074-48StripWells | MyBiosource | 48-Strip-Wells | EUR 550 |
Canine Progesterone Immunomodulatory Binding Factor 1 ELISA Kit |
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MBS054074-5x96StripWells | MyBiosource | 5x96-Strip-Wells | EUR 3420 |
Canine Progesterone Immunomodulatory Binding Factor 1 ELISA Kit |
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MBS054074-96StripWells | MyBiosource | 96-Strip-Wells | EUR 765 |