Photoelectrochemical immunoassay platform

immunograde

Photoelectrochemical immunoassay platform based on MoS 2 nanosheets built-in with gold nanostars for neuron-specific enolase assay

MoS2 nanosheets had been prepared by exfoliating MoS2 bulk crystals with ultrasonication in N-methylpyrrolidone and had been built-in with gold nanostars (AuNS) to fabricate an AuNS/MoS2 nanocomposite.
All nanomaterials had been characterised by transmission electron microscope, scanning electron microscope, ultraviolet-visible spectroscopy, X-ray diffraction, and X-ray photoelectron spectroscopy.
A0uNS/MoS2 nanocomposites had been coated onto a glassy carbon electrode (GCE) ground to assemble a nanointerface for immobilizing neuron-specific enolase antibody (anti-NSE) thus forming a photoelectrochemical immunoassay system.
AuNS can significantly promote the photoelectric conversion of MoS2 nanosheets bettering the effectivity for a photoelectrochemical assay.
Being illuminated with white gentle LED and controlling the potential at 0.05 V (vs. SCE), the photocurrent generated from anti-NSE(BSA)/AuNS/MoS2/GCE using 0.15 mol L-1 ascorbic acid as electron donor is likely to be recorded with amperometry and used as an output signal for NSE quantitative assay.
Beneath optimized experimental circumstances, the photocurrent variation for the affinity-binding NSE is proportional to the logarithm of NSE focus throughout the differ 5.Zero pg mL-1 to 1.5 ng mL-1 with a detection prohibit of three.5 pg mL-1 (S/N = 3).
The practicability of the PEC immunoassay system was evaluated by determining NSE in scientific serum samples. The recoveries ranged from 93.Zero to 103% for the dedication of NSE in serum samples with an unusual addition methodology.
The PEC immunoassay system possesses good accuracy for determining NSE in precise samples. Graphical abstract.
Key phrases: Gold nanostar; MoS2 nanosheet; Neuron-specific enolase; Photoelectrochemical immunoassay.
immunograde
immunograde

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Human Cytomegalovirus IgG / IgM, Toxoplasma IgG / IgM, Rubella IgG Rapid Test Kit

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Human Insulin Degrading Enzyme Chemiluminescent Immunoassay Kit

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Horse Toxoplasma gondii IgG(Toxo-IgG) ELISA Kit

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Camel Toxoplasma Gondii IgG(Toxo-IgG) ELISA Kit

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Human Toxoplasma gondii IgG(Toxo-IgG) ELISA Kit

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Human Toxoplasma gondii IgG (Toxo-IgG) ELISA Kit

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Cattle Toxoplasma gondii IgG (Toxo-IgG) ELISA Kit

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Chicken Toxoplasma gondii IgG(Toxo-IgG) ELISA Kit

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Human Toxoplasma gondii IgG (T. gondii IgG) ELISA Kit

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A microwell array structured ground plasmon resonance imaging gold chip for high-performance label-free immunoassay

 

Flooring plasmon resonance imaging (SPRi) offers a compelling methodology for high-throughput, real-time, and label-free biomolecular interaction analysis and immunoassays, nevertheless its effectivity suffers from restricted intrinsic sensitivity and low-contrast SPRi pictures.
Herein we report a high-performance SPRi chip that features patterned microwell array constructed by photolithography of adhesive polydopamine (PDA) skinny film on typical gold chip.
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The immunoassay effectivity of the as-designed SPRi chip is evaluated by using anti-ochratoxin A (anti-OTA) monoclonal antibody as a model aim.
The outcomes current that this microwell array structured gold chip shows ca. 18%-32% elevated signal depth than the typical gold chip when detecting anti-OTA at fully completely different concentrations, and the noise stays on the similar diploma, displaying enhanced intrinsic sensitivity.
Within the meantime, this microwell-structured chip affords clear and high-contrast SPRi pictures with well-defined sensing areas, which enormously facilitates the extraction and quantitative analysis of detection indicators whereas successfully suppressing the disturbance from background areas.

Antibody response in direction of SARS-CoV-2 spike protein and nucleoprotein evaluated by 4 automated immunoassays and three ELISAs

Objectives: To search out out the antibody response in direction of SARS-CoV-2 spike protein and nucleoprotein using 4 automated immunoassays and three ELISAs for the detection of entire Ig antibodies (Roche) or IgG (Abbott, Diasorin, Snibe, Euroimmun, Mikrogen) in COVID-19 victims.
Methods: Sensitivity and dynamic growth to seropositivity had been evaluated in 233 samples from 114 victims with affordable, excessive, or essential COVID-19 confirmed with PCR on nasopharyngeal swab. Specificity was evaluated in 113 samples collected sooner than January 2020 along with 24 samples from victims with non-SARS coronavirus an an infection.
Outcomes: Sensitivity for all assays was 100% [95% confidence interval: 83.7-100] Three weeks after onset of indicators. Specificity varied between 94.7% [88.7-97.8] and 100% [96.1-100]. Calculated on the cut-offs that corresponded to a specificity of 95% and 97.5%,
Roche had the very best sensitivity (85.0% [79.8-89.0] and 81.1% [76.6-85.7], p <0.05 in addition to vs. Abbott).
Seroconversion occurred on frequent 2 days earlier for Roche entire Ig anti-N and the 2 IgG anti-N assays (Abbott and Euroimmun) than for the 2 IgG anti-S assays (Diasorin and Euroimmun) (≥50% seroconversion day 9-10 vs. day 11-12, and p <0.05 for % seropositive victims day 9-10 to 17-18).
There was no very important distinction throughout the IgG antibody time to seroconversion between essential and non-critical victims.
Conclusions: Seroconversion occurred inside Three weeks after onset of indicators with all assays and on frequent 2 days earlier for assays detecting IgG or entire Ig anti-N than for IgG anti-S. The specificity of assays detecting anti-N was equivalent to anti-S and superb in a troublesome administration inhabitants.

Toxoplasma IgG Enzyme Immunoassay Test Kit

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Helicobacter Pylori IgG Enzyme Immunoassay Test Kit

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Cytomegalovirus (CMV) IgG Enzyme Immunoassay Test Kit

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Carcinoembryonic Antigen (CEA) Enzyme Immunoassay Test Kit

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Prostate Specific Antigen (PSA) Enzyme Immunoassay Test Kit

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Human Chorionic Gonadotropin (hCG) Enzyme immunoassay Test Kit

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EUR 379

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EUR 329

Troponin-I (Human Cardiac-Specific) Enzyme Immunoassay Test Kit

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EUR 681.12
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Human Estrone-3-Glucuronide (E1G)Enzyme Immunoassay ELISA Kit

QY-E05423 96T
EUR 433.2

Human PIBF(Progesterone-induced-blocking factor) ELISA Kit

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Human Insulin Degrading Enzyme Chemiluminescent Immunoassay Kit

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EUR 545
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Mouse Angiotensin I Converting Enzyme Chemiluminescent Immunoassay Kit

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EUR 545
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Human Angiotensin I Converting Enzyme Chemiluminescent Immunoassay Kit

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EUR 545
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Human Angiotensin I Converting Enzyme 2 Chemiluminescent Immunoassay Kit

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EUR 545
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Human Progesterone Immunomodulatory Binding Factor 1 ELISA Kit

IHUPIBF1KT each
EUR 799
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Progesterone / progesterone (PROG) ELISA Kit

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EUR 480

ELISA Kit for Progesterone Immunomodulatory Binding Factor 1 (PIBF1)

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TruStrip RDT Dog Progesterone Ovulation Rapid Test cards, 10/pk

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EUR 205.2

TruStrip RDT Dog Progesterone Ovulation Rapid Test cards, 25/pk

1856-RDT-25 1 pk
EUR 351.6

Human Progesterone Immunomodulatory Binding Factor 1 (PIBF1) ELISA Kit

DLR-PIBF1-Hu 96T
EUR 505
Description: tissue homogenates or other biological fluids.

Human Progesterone Immunomodulatory Binding Factor 1 (PIBF1) ELISA Kit

DLR-PIBF1-Hu-48T 48T
EUR 664.8
Description: A sandwich quantitative ELISA assay kit for detection of Human Progesterone Immunomodulatory Binding Factor 1 (PIBF1) in samples from tissue homogenates or other biological fluids.

Human Progesterone Immunomodulatory Binding Factor 1 (PIBF1) ELISA Kit

DLR-PIBF1-Hu-96T 96T
EUR 870
Description: A sandwich quantitative ELISA assay kit for detection of Human Progesterone Immunomodulatory Binding Factor 1 (PIBF1) in samples from tissue homogenates or other biological fluids.

Human PIBF1(Progesterone Immunomodulatory Binding Factor 1) ELISA Kit

ELK6860-48T 48T Ask for price
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PIBF1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PIBF1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PIBF1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PIBF1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Human PIBF1(Progesterone Immunomodulatory Binding Factor 1) ELISA Kit

ELK6860-96T 96T Ask for price
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PIBF1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PIBF1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PIBF1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PIBF1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Human Progesterone Immunomodulatory Binding Factor 1 (PIBF1) ELISA Kit

EKU06799-48T 48T
EUR 610.82

Human Progesterone Immunomodulatory Binding Factor 1 (PIBF1) ELISA Kit

EKU06799-5x96T 5x96T
EUR 4144.85

Human Progesterone Immunomodulatory Binding Factor 1 (PIBF1) ELISA Kit

EKU06799-96T 96T
EUR 872.6

Human Progesterone Immunomodulatory Binding Factor 1 (PIBF1) ELISA Kit

EKN47910-48T 48T
EUR 416.29

Human Progesterone Immunomodulatory Binding Factor 1 (PIBF1) ELISA Kit

EKN47910-5x96T 5x96T
EUR 2824.83

Human Progesterone Immunomodulatory Binding Factor 1 (PIBF1) ELISA Kit

EKN47910-96T 96T
EUR 594.7

Human Progesterone Immunomodulatory Binding Factor 1 (PIBF1) ELISA Kit

DL-PIBF1-Hu 96T
EUR 481
Description: tissue homogenates or other biological fluids.

Human Progesterone Immunomodulatory Binding Factor 1 (PIBF1) ELISA Kit

EK16081 96Т
EUR 768

Human Progesterone Immunomodulatory Binding Factor 1 (PIBF1) CLIA Kit

20-abx496098
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  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Human Progesterone Immunomodulatory Binding Factor 1 (PIBF1) ELISA Kit

20-abx152734
  • Ask for price
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  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Human Progesterone Immunomodulatory Binding Factor 1 (PIBF1) ELISA Kit

RD-PIBF1-Hu-48T 48T
EUR 493.5
Description: tissue homogenates and other biological fluids.

Human Progesterone Immunomodulatory Binding Factor 1 (PIBF1) ELISA Kit

RD-PIBF1-Hu-48Tests 48 Tests
EUR 675.6

Human Progesterone Immunomodulatory Binding Factor 1 (PIBF1) ELISA Kit

RD-PIBF1-Hu-96T 96T
EUR 705
Description: tissue homogenates and other biological fluids.

Human Progesterone Immunomodulatory Binding Factor 1 (PIBF1) ELISA Kit

RD-PIBF1-Hu-96Tests 96 Tests
EUR 939.6

Human Progesterone Immunomodulatory Binding Factor 1 (PIBF1) ELISA Kit

SEN433Hu-10x96wellstestplate 10x96-wells test plate
EUR 6227.58
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Progesterone Immunomodulatory Binding Factor 1 (PIBF1) in Tissue homogenates and other biological fluids.

Human Progesterone Immunomodulatory Binding Factor 1 (PIBF1) ELISA Kit

SEN433Hu-1x48wellstestplate 1x48-wells test plate
EUR 618.04
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Progesterone Immunomodulatory Binding Factor 1 (PIBF1) in Tissue homogenates and other biological fluids.

Human Progesterone Immunomodulatory Binding Factor 1 (PIBF1) ELISA Kit

SEN433Hu-1x96wellstestplate 1x96-wells test plate
EUR 831.48
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Progesterone Immunomodulatory Binding Factor 1 (PIBF1) in Tissue homogenates and other biological fluids.

Human Progesterone Immunomodulatory Binding Factor 1 (PIBF1) ELISA Kit

SEN433Hu-5x96wellstestplate 5x96-wells test plate
EUR 3381.66
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Progesterone Immunomodulatory Binding Factor 1 (PIBF1) in Tissue homogenates and other biological fluids.

Human Progesterone Immunomodulatory Binding Factor 1 (PIBF1) ELISA Kit

4-SEN433Hu
  • Ask for price
  • Ask for price
  • Ask for price
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Progesterone Immunomodulatory Binding Factor 1 (PIBF1) in samples from Tissue homogenates and other biological fluids. with no significant corss-reactivity with analogues from other species.

Human Progesterone Immunomodulatory Binding Factor 1 (PIBF1) ELISA Kit

RDR-PIBF1-Hu-48T 48T
EUR 518.18
Description: tissue homogenates and other biological fluids.

Human Progesterone Immunomodulatory Binding Factor 1 (PIBF1) ELISA Kit

RDR-PIBF1-Hu-48Tests 48 Tests
EUR 706.8

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